The ability to chemically synthesize single-stranded oligonucleotides has had a profound impact on research and medicine. Yet existing strategies have their limitations. One critical limitation is the...
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Transfection is the process of introducing nucleic acids into cells. Two main strategies are viral (utilizing viruses as vectors) and non-viral. Non-viral methods are less efficient but attractive for...
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DNA synthesis is evolving from chip-based synthesis to a method where oligomers are synthesized and subsequently harvested so this genetic material can be used in a construction kit-type system. This ...
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Enzymes of the glutathione S-transferase (GST) family are present in both eukaryotes and prokaryotes. As much as 10 percent of the cytosolic protein in some mammalian organs can consist of GSTs.
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Restriction enzymes are used widely to cleave double-stranded DNA. However, a given restriction enzyme can only cleave a DNA molecule at the specific nucleotide sequence that corresponds to the recogn...
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Effective isolation of nucleic acids from biological samples is an essential prerequisite for efficient downstream amplification, detection and quantification of specific genetic sequences via quantit...
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Molecule-molecule interactions, including interactions between proteins, nucleic acids and other small molecules, are important in a variety of cellular events. A number of physical, molecular biologi...
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Now that complete sequence information is available for many organisms, researchers are focusing on the analysis of single genomic DNA molecules. A key issue in this analysis is how to unravel and mar...
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Microbial genome sequencing projects uncover large numbers of new genes. Functional analyses of these genes require targeted modifications of particular DNA sequences in their chromosomal locations us...
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Because they retain large DNA inserts over many generations, bacterial artificial chromosomes (BACs) are popular vectors for genetic and expression analyses of genomic DNA libraries; however, their us...
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Promoters are genetic regulatory elements that drive gene expression in cells under certain conditions. One way to identify promoters is to use a promoter-trap vector, which is a plasmid containing a ...
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The low mobility of bacterial transposons, such as Tn5, makes it difficult for researchers to detail the molecular transposition process and to exploit transposition for uses such as the development o...
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Cationic lipid-mediated delivery of nucleic acids and proteins may replace virus-mediated transfection of exogenous DNA for many applications. However, current lipid-based carriers provide limited con...
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The normal growth and development of tissue requires a complex interplay of multiple signals from multiple genes in well-defined locations, leading to the controlled differentiation of precursor cells...
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RNA polymerase, the enzyme responsible for making RNA from a DNA or RNA template, is one of the most important proteins in all life forms.
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