UW-Madison and Morgridge researchers have developed new methods and systems for complete structural characterization of biological samples. The researchers combined mass spectrometry technologies with...
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A UW-Madison researcher proposes a new strategy that uses small, stress-response proteins to preserve fragile samples during cryo-EM SPA sample preparation. The figure below illustrates the general sa...
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UW-Madison and Morgridge Institute for Research researchers have created the first high throughput instrument to integrate single cell autofluorescence lifetimes with single-cell transcription (scRNA-...
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UW-Madison researchers have developed a unique inherent autofluorescent image-based classifiers neutrophil activation. The specific autofluorescence signatures are used to identify neutrophil activati...
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UW-Madison researchers have developed a unique inherent fluorescent image-based classifiers for B cell and NK cell activation. The specific autofluorescence signatures are used to identify B cell acti...
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UW-Madison researchers have developed a novel profluorophore that binds to lithium ions with high specificity, inducing a fluorescent signal. The fluorescent indicator can be seamlessly integrated int...
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Fluorescent microscopes, including wide-field, confocal and super-resolution, are widely used instruments in a variety of research fields but require routine calibration and maintenance. Calibration r...
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Single particle cryo-electron microscopy (cryo-EM) is a powerful tool for providing 3-D structural information on non-crystalline specimens. Cryo-EM approaches atomic level resolution, enabling many n...
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Single particle cryo-electron microscopy is a powerful tool for providing 3-D structural information on non-crystalline specimens. Cryo-EM approaches atomic level resolution, enabling many new biologi...
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Single particle cryo-electron microscopy (cryo-EM) is a powerful tool for providing 3-D structural information on non-crystalline specimens. Cryo-EM approaches atomic level resolution, enabling many n...
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Light microscopy is a well-established tool to study protein dynamics and protein functions in fixed and living cells. One limitation of light microscopy is that its diffraction limit (optical resolut...
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UW-Madison and Morgridge Institute researchers have identified developed an improved method for depositing samples on electron microscopy (EM) grids. The technology leverages a modified mass spectrome...
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A method (100) can be provided to determine the throughput speed v of at least one pore (1), comprising: • feeding (110), using a driving force F, a filiform calibration element (2) through the pore...
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A new cancer treatment being studied at a few major centers including UW Health is CAR T (Chimeric Antigen Receptor T cell) therapy. CAR T therapy uses a patient's own cells and 'reengineers&#...
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The importance of T cells in immunotherapy is driving the development of technologies to better characterize T cells and improve therapeutic efficacy. One specific objective is assessing antigen-induc...
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Regenerative medicine requires a practical, high throughput method of assessing the cellular components of multicellular entities before transplantation. Such a method would have many clinical and res...
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Label-free sensing methods could enable detection and characterization of chemical species at the single molecule level without the aid of probes or modifications to the target of interest. Such capab...
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Single molecule spectroscopy and microscopy – a popular tool for biological, biophysical and chemical characterization – generally requires the use of fluorescent molecules. Researchers at UW–M...
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Studying molecular properties in complex chemical and biological samples, including live cells, requires sufficient spatial resolution and contrast between different substructures. To achieve enhanced...
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Atomically sharp micro-fabricated tips are used in atomic force microscopy (AFM) for scanning near-field microwave microscopy (SNMM) to create an image of the tiny topography on or under a surface. Th...
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A whispering gallery mode (WGM) is a type of electromagnetic wave that travels around a concave surface. Such waves can propagate at the edge of a very small structure called an optical microcavity. W...
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Intrinsic and extrinsic fluorescent markers can help surgeons target and rapidly identify tumorous tissue. The signals given off by these markers are relatively faint and normally viewed with a fluore...
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Confocal microscopy is used to view biological tissue through intervening tissues or structures. Multiphoton confocal microscopy uses a focused beam of photons to provide high-resolution images of tis...
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The atomic force microscope (AFM), one of many types of scanned-proximity probe microscopes, images a sample by lightly touching it with a probe tip attached to the end of an extremely flexible leaf s...
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Although detecting high-frequency magnetic fields is important in MRI, NMR and magnetic resonance force microscopes, it is more difficult to design magnetic field sensors at high frequencies (greater ...
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