The commercially available E. coli strain BL21 λDE3 is commonly used for inducibly overexpressing recombinant proteins. Many derivatives of BL21 cells have been developed and commercialized, with pro...
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A non-displasive pipette is provided. The non-displasive pipette is configured for connection to a tip having a fluid therein. The tip includes an orifice to allow for the discharge of the fluid there...
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Devices and methods for using changes in the configuration of micrometer sized dispersed liquid crystal domains to detect or quantify analytes in a test sample, including endotoxin lipopolysaccharide ...
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Gram-negative bacterial infections are an ongoing challenge to health care providers worldwide. Detection, treatment and control of such pathogens have gained increasing importance in the context of a...
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In animals, plants and other eukaryotic organisms, typically one protein is translated from each mRNA. Usually, translation can start only at one end of the mRNA strand. In contrast, several viruses ...
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Boronic acids (i.e., compounds that have a single boron-carbon bond) are one of the most useful functional groups in organic chemistry and chemical biology. They have applications in polymer sciences,...
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Enantiomers are pairs of compounds that have the same chemical formula but different spatial structures (like your left and right hand, they are non-superimposable mirror images of each other). This i...
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Removal of virus is essential for the manufacture of safe biotechnology-derived products such as therapeutic monoclonal antibodies. Currently, virus is removed using ion exchange chromatographic resin...
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Removal of virus is essential for the manufacture of safe biotechnology-derived products such as therapeutic monoclonal antibodies. Currently, virus is removed using ion exchange chromatographic resin...
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Bacterial production of recombinant proteins is used commonly by researchers and commercial entities to manufacture a large variety of proteins. However, many proteins are not produced efficiently in ...
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UW–Madison researchers previously developed a method of using traceless Staudinger ligation to chemically synthesize proteins (see WARF reference number P00315US).  This method is based on the Stau...
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Acyl carrier protein (ACP) is a small lipid carrier protein involved in fatty acid biosynthesis and in the solubilization of fats to make them metabolically available. The molecule is difficult to obt...
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Epitope tagging provides an improved method of protein immunopurification. The technique involves adding a small peptide epitope to a protein of interest through recombinant DNA techniques. The recomb...
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Phosphoglucomutase (PGM) is a glycolytic enzyme that converts glucose 1-phosphate to glucose 6-phosphate. This enzyme plays a major role in directing the flow of carbon toward either anabolic or catab...
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Foldamers are synthetic protein oligomers with discrete and predictable folding properties akin to those of natural proteins. They provide molecular tools to investigate large-molecule interactions su...
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Much of the research on RNA transcription is focused on the action of RNA polymerases. Thus, improved methods for purifying and characterizing RNA polymerase greatly enhance studies of transcription. ...
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Purification of biological components, including viruses, plasmids, proteins and small molecules, typically relies on chromatography for most of the separation steps. Membrane filtration cascades prov...
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Although the yeast Saccharomyces cerevisiae is often used to express foreign proteins for industrial or therapeutic uses, production of these heterologous proteins is far from optimal. Current methods...
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