Pluripotent Stem Cells
Clonal Culture of Human Pluripotent Stem Cells
WARF: P08168US
Inventors: James Thomson, Veit Bergendahl
The Wisconsin Alumni Research Foundation (WARF) is seeking commercial partners interested in developing a method to increase the cloning efficiency of stem cell culture using kinase inhibitors.
Overview
For researchers, cloning human embryonic stem (ES) cells is challenging because it requires growing new colonies from a single cell. One difficulty is very low cloning efficiency – less than 0.1 percent when growing the cells in defined, xenogen-free culture conditions.
The Invention
UW–Madison researchers have developed a method to boost the cloning efficiency of any effective ES cell culture medium. The researchers screened small molecules and found they could increase efficiency rates using kinase enzyme inhibitors, e.g., protein kinase A/C/G inhibitors or a Rho-associated kinase (ROCK) inhibitor.
Applications
- Culturing human embryonic or induced pluripotent stem cells
Key Benefits
- Improves cloning efficiency
- Effective and economical
Additional Information
For More Information About the Inventors
Tech Fields
For current licensing status, please contact Andy DeTienne at [javascript protected email address] or 608-960-9857