Pluripotent Stem Cells
Generating Vasculogenic Cell Populations from Human Stem Cells
WARF: P130364US02
Inventors: Igor Slukvin, Akhilesh Kumar
The Wisconsin Alumni Research Foundation (WARF) is seeking commercial partners interested in developing a method for differentiating human pluripotent stem cells to pericytes and smooth muscle cells.
Overview
Human pluripotent stem cells (hPSCs), either embryonic or induced, provide access to the earliest stages of human development. They also offer a starting point for deriving large numbers of vasculogenic cells that could be utilized for therapeutic tissue engineering, e.g., creating new blood vessels to treat peripheral artery disease.
To exploit this potential, methods are needed for guiding pluripotent cell differentiation in vitro and developing scalable sources of such cells.
To exploit this potential, methods are needed for guiding pluripotent cell differentiation in vitro and developing scalable sources of such cells.
The Invention
UW–Madison researchers have developed a method for generating substantially pure populations of vasculogenic cells (i.e., pericytes and smooth muscle cells) from induced pluripotent stem cells following their differentiation into mesenchymal colony-forming progenitors, called mesenchymoangioblasts (MABs).
The process includes culturing the progenitors in a serum-free medium under conditions that promote differentiation to MABs. Subsequently, the MABs are cultured in medium containing PDGFBB to obtain pericytes, or sphingosylphosphorylcholine (SPC) and transforming growth factor beta (TGFβ) to obtain smooth muscle cells.
The process includes culturing the progenitors in a serum-free medium under conditions that promote differentiation to MABs. Subsequently, the MABs are cultured in medium containing PDGFBB to obtain pericytes, or sphingosylphosphorylcholine (SPC) and transforming growth factor beta (TGFβ) to obtain smooth muscle cells.
Applications
- Producing pericytes and vascular smooth muscle cells from hPSCs
- Regenerative medicine
- Vascular tissue engineering (e.g., creating blood vessels in vitro or in vivo to revascularize damaged tissue or treat peripheral artery disease)
Key Benefits
- Method uses a well-defined mesodermal progenitor to produce pericytes and smooth muscle cells from pluripotent stem cells.
- Scalable
- Provides very specific surface markers for pericytes from pluripotent stem cells
Additional Information
For More Information About the Inventors
Tech Fields
For current licensing status, please contact Andy DeTienne at [javascript protected email address] or 608-960-9857