Clean Technology
POLYMER-DEGRADING ENZYMES USING YEAST DISPLAY AND METHODS OF USE THEREOF
WARF: P240043US02
Inventors: Jeffrey Martell, Mario Cribari, Maxwell Unger, Ashley Ogorek
The Invention
UW-Madison researchers disclose an ultrahigh-throughput directed evolution platform. The platform leverages yeast display, a specially designed modular molecular probe, and fluorescence-based cell sorting (FACS). In the platform, individual yeast cells display distinct mutant enzymes and enzymatic activity is detected by a change in fluorescence upon cleavage of a PET-mimicking synthetic probe. Upon identification, the highly active mutants are isolated using FACS and are subsequently characterized using DNA sequencing. The platform allowed the inventors to experimentally screen 40 million plastic-degrading mutant enzymes.
Additional Information
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Tech Fields
For current licensing status, please contact Jennifer Gottwald at [javascript protected email address] or 608-960-9854