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UW-Madison Researchers have developed a microfluidic device for profiling cell types, evaluating multiple functional read-outs in an efficient, user-friendly high-throughput manner. The device allows a user to evaluate multiple functional readouts using smaller volumes of blood than prior devices/methods and leverages the traditional 384 well plate design making it compatible with a wide range of instruments such as microscopes and plate readers. It is comprised of an interconnected network of microchannels connecting adjacent wells that contain reaction-specific buffers to monitor multiple aspects of cell response. The system does not require specialized pumping equipment and is highly compatible with many common laboratory instruments.