The ability to unambiguously mark a cell’s genotype is essential for studies in which genetically distinct cell populations must be distinguished from one another in vivo.
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Microorganisms adapted to grow in exceptionally harsh environments, such as hot springs and arctic soils, are a potentially vast source of novel metabolic processes, antibiotics, enzymes and other pro...
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The enzyme RNA polymerase II synthesizes precursors to mRNAs and small nuclear RNAs. Sequence analysis of the largest subunit of RNA polymerase II has revealed an unusual heptapeptide repeat at the mo...
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The study of full-length cDNA clones is important for determining the structure and function of genes.
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CREB is a transcription factor that has been implicated as a critical regulator of neuronal function and survival. Knockout mice lacking CREB exhibit a disease similar to ataxia telangiectasia, a neur...
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Titin is a very large cardiac protein that determines the resting tension of the heart in rats. Isoforms of titin are generated by alternative splicing of a single titin gene. Titin in embryonic rat h...
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Cultured microorganisms produce an extraordinary array of structurally diverse and valuable organic compounds; however, microbes that can be brought into culture by standard techniques represent only ...
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Three hybrid screens are used to detect RNA-protein interactions.  A problem with three hybrid screens has been high background due to some cDNAs binding and activating transcription in the absence o...
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The gene HERG1 encodes a potassium-selective ion channel important in maintaining normal cardiac rhythm and cell growth.
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The liver detoxifies many of the drugs and toxins in blood, which poses a problem in drug development and testing because drugs are rapidly cleared from the system. In the mammalian fetus, the liver i...
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HERG (human ether-a-go-go-related gene) encodes a potassium ion channel important for maintaining normal cardiac rhythm and cell growth.
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CpG islands are areas of DNA containing a high CpG content, which often correspond to promoter regions.
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The human ERG gene encodes a potassium channel that is expressed in the heart. Mutations in the HERG channel are a common cause of long QT syndrome, a disorder associated with delayed cardiac repolari...
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Cardiac IKr channels are targets associated with inherited and acquired long QT syndrome (LQTS), a disorder that can lead to ventricular arrhythmias. Previously, IKr channels were thought to be compos...
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The human heart sodium channel beta1 subunit (SCNB1) is a protein encoded by a single gene. It interacts with the alpha subunit to alter the functional properties of the channel. In addition, SCNB1 is...
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Cultured microorganisms produce an extraordinary array of structurally diverse and valuable organic compounds; however, microbes that can be cultured using standard techniques represent only a small f...
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Most biochemical interactions are studied in solutions containing dilute concentrations of molecules. However, the complexity of the cellular environment makes it difficult to compare the results of s...
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No well-characterized prostate stromal cell lines currently exist.
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The six mammalian actin genes encode a highly conserved family of cytoskeletal protein isoforms important in cell structure, mechanical stability, and motility. Actin isoforms exhibit tissue-specific ...
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The human either-a-go-go related gene (HERG1 or KCNH2) potassium channel is critical to maintaining normal cardiac rhythm. Because unintended block of HERG channel activity by drugs can cause long QT ...
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Because they retain large DNA inserts over many generations, bacterial artificial chromosomes (BACs) are popular vectors for genetic and expression analyses of genomic DNA libraries; however, their us...
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Promoters are genetic regulatory elements that drive gene expression in cells under certain conditions. One way to identify promoters is to use a promoter-trap vector, which is a plasmid containing a ...
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Microorganisms adapted to grow in exceptionally harsh environments, such as hot springs and arctic soils, are a potentially vast source of novel metabolic processes, antibiotics, enzymes and other pro...
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Microorganisms adapted to grow in exceptionally harsh environments, such as hot springs and arctic soils, are a potentially vast source of novel metabolic processes, antibiotics, enzymes and other pro...
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Humans with mutations in the Adenomatous Polyposis Coli (APC) gene develop hundreds or even thousands of adenomas in the colon, which often lead to colorectal cancer. Mouse models of human intestinal ...
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